
Short-term effects of cover crops on soil properties and the abundance of N-cycling genes in citrus agroecosystems
Limited information is available on the influence of cover crops (CCs) on soil nutrient cycling and the abundance of bacterial and archaeal communities, nitrogen (N)-fixers, nitrifiers, and denitrifiers in tree crops. We examined the effect of replacing the traditional weedy inter-row middle of a commercial citrus orchard in Florida with two different mixtures of CCs: legumes and non-legumes (LG + NL) and non-legumes only (NL). A no-treatment/grower standard was used as a control (GSC). After one year of CCs, the use of NL and LG + NL CCs significantly increased soil carbon (C) availability in the row middles compared to GSC as shown by increases in permanganate oxidizable C, and to a lesser extent, soil organic matter. Treatment with LG + NL significantly increased soil ammonium content compared to the NL and GSC treatments. Improved C cycling in soils treated with CCs was related to increased abundance of bacteria and archaea. Changes in the size of the soil N pool were accompanied by increases in the abundance of N-fixation (nifH) and nitrification (amoA) genes in soils treated with LG + NL, likely due to greater N-mineralization and N-fixation by legumes.