The efficacy of applying biocontrol agents, chemical fungicide and nematicide as protective treatments against the soilborne parasites, Fusarium spp. (Fusaria) and citrus nematode Tylenchulus semipenetrans Cobb was evaluated. The experiment took place under field conditions in a citrus orchard cultivated with 16-year-old sweet orange (Citrus sinensis L.) osbech cv. Valencia trees grafted on sour orange (C. aurantium L.) rootstock during the growing season November 2006/ October 2007. This orchard is located at Bader district, Behera governorate, Egypt. The populations of soil fauna and flora under trees canopy were examined just before treatment, and 1, 3, 6, 9 and 12 months after the treatment application. A visual inspection for the appearance of symptoms related to Fusarium or nematode infection on treated and untreated citrus trees was carried out periodically every two weeks throughout the experimental period.