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Tag: Candidatus Liberibacter Asiaticus

25 May 2021
HLB
0 Comments By Firstfruits Consulting Website Articles, News , , ,

CRISPR/Cas technology could enable early diagnosis of devastating citrus disease

Scientists from Penn State and the U.S. Department of Agriculture have developed a diagnostic test that enables early detection of Huanglongbing (HLB) by detecting the presence of the bacterium that causes the disease, i.e. Candidatus Liberibacter asiaticus (CLas). Early detection of CLas is vital in controlling the spread of HLB because trees that have been infected by CLas can serve as a disease reservoir for years before symptoms are visible.

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26 May 2020
Citrus Greening
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Assessment of unconventional antimicrobial compounds for the control of ‘Candidatus Liberibacter asiaticus’, the causative agent of citrus greening disease.

In this study, newly identified small molecules were examined for efficacy against ‘Candidatus Liberibacter asiaticus’ in commercial groves of sweet orange (Citrus sinensis) and white grapefruit (Citrus paradisi) trees. We used benzbromarone and/or tolfenamic acid delivered by trunk injection. We evaluated safety and efficacy parameters by performing RNAseq of the citrus host responses, 16S rRNA gene sequencing to characterize citrus-associated microbial communities during treatment, and qRT-PCR as an indirect determination of ‘Ca. L. asiaticus’ viability. Analyses of the C. sinensis transcriptome indicated that each treatment consistently induced genes associated with normal metabolism and growth, without compromising tree viability or negatively affecting the indigenous citrus-associated microbiota.

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